Primary Airway Ciliated Epithelial Cell Services

Cytoptics provides to industry, academia and government, the evaluation of targeted agents on ciliary beat frequency using our Cilscreen™ and Ciliascope™ technologies.

The effects of the selected agents are tested using primary cultures of canine, ovine or porcine ciliated airway epithelial cells, CiliScreen™ . These ciliated airway epithelial cells sustain robust, synchronous beating for weeks. The cell morphology resembles those found in in vivo conditions.

Measurements of Ciliary beat frequency, CBF, are made with Ciliascope™ . Ciliascope™ is an epi-illumination inverted microscope specifically designed for the real-time measurement of ciliary beat frequency (CBF). Ciliascope™ uses dynamic laser light scattering and nonstationary signal analysis to measure, display, and record CBF as the experiment is being performed. Ciliascope™ measures CBF in every 2 to 5 seconds. Thus responses of ciliary beat to the test agent can be measured in very short (seconds) and long (hours) times frames according the agent's mode of action.

Applications of our Airway Epithelial Cell Products and Services

•  Airway disease research

•  Drug discovery and development

•  Cost effective alternative to pre-clinical whole-animal screening

•  Toxicology

Cytoptics scientific staff will help you define your needs, plan your studies, perform the experiments, provide the raw and analyzed data and assist in its interpretation.

For more information, please contact: info@cyotoptics.com or call 760 744 0036 or fax 760 744 0033 .

Acknowledgements: The research and development of the cultured ciliated cells were supported by R43 & R44 HL 67595 from NIH . The research and development of the Ciliascope™ were supported by R43 & R44 HL 59126 also from NIH